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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
07/03/2023 |
Actualizado : |
07/03/2023 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
MUSSIO, P.; MARTÍNEZ, I.; LUZARDO, S.; NAVARRO, A.; LEOTTA, G.; VARELA, G. |
Afiliación : |
PAULA MUSSIO, Departamento de Microbiología, Laboratorio Tecnológico del Uruguay, Montevideo, Uruguay; INÉS MARTÍNEZ, Latitud, Fundación LATU, Montevideo, Uruguay; SANTIAGO FELIPE LUZARDO VILLAR, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ARMANDO NAVARRO, Departamento de Salud Pública, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, Mexico; GERARDO LEOTTA, Instituto de Ciencia y Tecnología de Sistemas Alimentarios Sustentables, UEDD INTA-CONICET, Buenos Aires, Argentina; GUSTAVO VARELA, Departamento de Bacteriología y Virología, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay. |
Título : |
Phenotypic and genotypic characterization of Shiga toxin-producing Escherichia coli strains recovered from bovine carcasses in Uruguay. |
Complemento del título : |
Original research. |
Fecha de publicación : |
2023 |
Fuente / Imprenta : |
Frontiers in Microbiology, 2023, volume 14, article 1130170. OPEN ACCESS. doi: https://doi.org/10.3389/fmicb.2023.1130170 |
DOI : |
10.3389/fmicb.2023.1130170 |
Idioma : |
Inglés |
Notas : |
Article history: Received 22 December 2022; Accepted 13 February 2023; Published 06 March 2023. -- Correspondence authors: Paula Mussio, email: paumussio@gmail.com ; Gustavo Varela, email: gvarela@higiene.edu.uy -- Edited by: Vinicius Castro, University of Lethbridge, Canada. -- Specialty Section:
This article was submitted to Food Microbiology, a section of the journal Frontiers in Microbiology. -- This article is part of the Research Topic
From Farm Gate to Food Plate: Current Challenges in Foodborne Microorganism Detection, Epidemiology, and Genetic Diversity (https://www.frontiersin.org/research-topics/49842/from-farm-gate-to-food-plate-current-challenges-in-foodborne-microorganism-detection-epidemiology-and-genetic-diversity#articles ). -- LICCENSE: This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY), (http://creativecommons.org/licenses/by/4.0/ ). -- Supplementary material: The Supplementary material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmicb.2023.1130170/full#supplementary-material . -- FUNDING: This work was supported by Agencia Nacional de Investigación e Innovación (ANII), Fondo Sectorial Innovagro - Inocuidad, Grant: FSA_I_2017_1_140224. |
Contenido : |
Introduction: Shiga toxin-producing Escherichia coli (STEC) is a zoonotic pathogen that cause food-borne diseases in humans. Cattle and derived foodstuffs play a known role as reservoir and vehicles, respectively. In Uruguay, information about the characteristics of circulating STEC in meat productive chain is scarce. The aim was to characterize STEC strains recovered from 800 bovine carcasses of different slaughterhouses. Methods: To characterize STEC strains we use classical microbiological procedures, Whole Genome Sequencing (WGS) and FAO/WHO risk criteria. |
Palabras claves : |
Bovine carcasses; Shiga toxin-producing Escherichia coli (STEC); URUGUAY; Virulence factors; Whole Genome Sequencing (WGS); Zoonotic pathogen. |
Asunto categoría : |
L40 Estructura animal |
URL : |
https://www.frontiersin.org/articles/10.3389/fmicb.2023.1130170/pdf
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Marc : |
LEADER 02780naa a2200277 a 4500 001 1063967 005 2023-03-07 008 2023 bl uuuu u00u1 u #d 024 7 $a10.3389/fmicb.2023.1130170$2DOI 100 1 $aMUSSIO, P. 245 $aPhenotypic and genotypic characterization of Shiga toxin-producing Escherichia coli strains recovered from bovine carcasses in Uruguay.$h[electronic resource] 260 $c2023 500 $aArticle history: Received 22 December 2022; Accepted 13 February 2023; Published 06 March 2023. -- Correspondence authors: Paula Mussio, email: paumussio@gmail.com ; Gustavo Varela, email: gvarela@higiene.edu.uy -- Edited by: Vinicius Castro, University of Lethbridge, Canada. -- Specialty Section: This article was submitted to Food Microbiology, a section of the journal Frontiers in Microbiology. -- This article is part of the Research Topic From Farm Gate to Food Plate: Current Challenges in Foodborne Microorganism Detection, Epidemiology, and Genetic Diversity (https://www.frontiersin.org/research-topics/49842/from-farm-gate-to-food-plate-current-challenges-in-foodborne-microorganism-detection-epidemiology-and-genetic-diversity#articles ). -- LICCENSE: This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY), (http://creativecommons.org/licenses/by/4.0/ ). -- Supplementary material: The Supplementary material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmicb.2023.1130170/full#supplementary-material . -- FUNDING: This work was supported by Agencia Nacional de Investigación e Innovación (ANII), Fondo Sectorial Innovagro - Inocuidad, Grant: FSA_I_2017_1_140224. 520 $aIntroduction: Shiga toxin-producing Escherichia coli (STEC) is a zoonotic pathogen that cause food-borne diseases in humans. Cattle and derived foodstuffs play a known role as reservoir and vehicles, respectively. In Uruguay, information about the characteristics of circulating STEC in meat productive chain is scarce. The aim was to characterize STEC strains recovered from 800 bovine carcasses of different slaughterhouses. Methods: To characterize STEC strains we use classical microbiological procedures, Whole Genome Sequencing (WGS) and FAO/WHO risk criteria. 653 $aBovine carcasses 653 $aShiga toxin-producing Escherichia coli (STEC) 653 $aURUGUAY 653 $aVirulence factors 653 $aWhole Genome Sequencing (WGS) 653 $aZoonotic pathogen 700 1 $aMARTÍNEZ, I. 700 1 $aLUZARDO, S. 700 1 $aNAVARRO, A. 700 1 $aLEOTTA, G. 700 1 $aVARELA, G. 773 $tFrontiers in Microbiology, 2023, volume 14, article 1130170. OPEN ACCESS. doi: https://doi.org/10.3389/fmicb.2023.1130170
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INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
08/03/2023 |
Actualizado : |
15/03/2023 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
RAMOS, Z.; GARRICK , D. J.; BLAIR, H. T.; VERA, B.; CIAPPESONI, G.; KENYON, P. R. |
Afiliación : |
ZULLY MARGOT RAMOS ALVEZ, School of Agriculture and Environment, Massey University, Palmerston North 4410, New Zealand; DORIAN J. GARRICK, School of Agriculture and Environment, Massey University, Palmerston North 4410, New Zealand; HUGH T. BLAIR, School of Agriculture and Environment, Massey University, Palmerston North 4410, New Zealand; BRENDA VERA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS GABRIEL CIAPPESONI SCARONE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PAUL R. KENYON, School of Agriculture and Environment, Massey University, Palmerston North 4410, New Zealand. |
Título : |
Genomic regions associated with wool, growth and reproduction traits in Uruguayan Merino Sheep. |
Fecha de publicación : |
2023 |
Fuente / Imprenta : |
Genes, 2023, volume 14, issue 1, article 167. OPEN ACCESS. doi: https://doi.org/10.3390/genes14010167 |
ISSN : |
2073-4425 |
DOI : |
10.3390/genes14010167 |
Idioma : |
Inglés |
Notas : |
Article history: Received 13 December 2022; Revised 29 December 2022; Accepted 4 January 2023; Published 7 January 2023. -- Correspondence author: Zully Ramos, email: z.ramosalvez@massey.ac.nz -- Academic Editor: Qiuyue Liu -- This article belongs to the Section Animal Genetics and Genomics (https://www.mdpi.com/journal/genes/sections/Animal_Genetics_Genomics ) -- FUNDING: his research was funded by the Regional Consortium for Innovation in Ultrafine Wool (CRILU), the European Union?s Horizon 2020 research and innovation program under the grant agreement n°772787 (Smarter) and the National Institute of Agricultural Research of Uruguay (INIA_CL_38: Rumiar). This study was supported by two Ph.D. scholarships (from the National Agency for Investigation and Innovation of Uruguay, ANII, and Massey University, New Zealand), awarded to Zully Ramos. -- LICENSSE: Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). --
Supplementary Materials The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/genes14010167/s1, Table S1: Enrichment analysis. |
Contenido : |
The aim of this study was to identify genomic regions and genes associated with the fiber diameter (FD), clean fleece weight (CFW), live weight (LW), body condition score (BCS), pregnancy rate (PR) and lambing potential (LP) of Uruguayan Merino sheep. Phenotypic records of approximately 2000 mixed-age ewes were obtained from a Merino nucleus flock. Genome-wide association studies were performed utilizing single-step Bayesian analysis. For wool traits, a total of 35 genomic windows surpassed the significance threshold (PVE > 0.25%). The proportion of the total additive genetic variance explained by those windows was 4.85 and 9.06% for FD and CFW, respectively. There were 42 windows significantly associated with LWM, which collectively explained 43.2% of the additive genetic variance. For BCS, 22 relevant windows accounted for more than 40% of the additive genetic variance, whereas for the reproduction traits, 53 genomic windows (24 and 29 for PR and LP, respectively) reached the suggestive threshold of 0.25% of the PVE. Within the top 10 windows for each trait, we identified several genes showing potential associations with the wool (e.g., IGF-1, TGFB2R, PRKCA), live weight (e.g., CAST, LAP3, MED28, HERC6), body condition score (e.g., CDH10, TMC2, SIRPA, CPXM1) or reproduction traits (e.g., ADCY1, LEPR, GHR, LPAR2) of the mixed-age ewes. Copyright: © 2023 by the authors |
Palabras claves : |
Body condition score; Fiber diameter; Gene; GWAS; Reproduction; Sheep. |
Asunto categoría : |
L10 Genética y mejoramiento animal |
URL : |
https://www.mdpi.com/2073-4425/14/1/167/pdf
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Marc : |
LEADER 03484naa a2200289 a 4500 001 1063971 005 2023-03-15 008 2023 bl uuuu u00u1 u #d 022 $a2073-4425 024 7 $a10.3390/genes14010167$2DOI 100 1 $aRAMOS, Z. 245 $aGenomic regions associated with wool, growth and reproduction traits in Uruguayan Merino Sheep.$h[electronic resource] 260 $c2023 500 $aArticle history: Received 13 December 2022; Revised 29 December 2022; Accepted 4 January 2023; Published 7 January 2023. -- Correspondence author: Zully Ramos, email: z.ramosalvez@massey.ac.nz -- Academic Editor: Qiuyue Liu -- This article belongs to the Section Animal Genetics and Genomics (https://www.mdpi.com/journal/genes/sections/Animal_Genetics_Genomics ) -- FUNDING: his research was funded by the Regional Consortium for Innovation in Ultrafine Wool (CRILU), the European Union?s Horizon 2020 research and innovation program under the grant agreement n°772787 (Smarter) and the National Institute of Agricultural Research of Uruguay (INIA_CL_38: Rumiar). This study was supported by two Ph.D. scholarships (from the National Agency for Investigation and Innovation of Uruguay, ANII, and Massey University, New Zealand), awarded to Zully Ramos. -- LICENSSE: Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). -- Supplementary Materials The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/genes14010167/s1, Table S1: Enrichment analysis. 520 $aThe aim of this study was to identify genomic regions and genes associated with the fiber diameter (FD), clean fleece weight (CFW), live weight (LW), body condition score (BCS), pregnancy rate (PR) and lambing potential (LP) of Uruguayan Merino sheep. Phenotypic records of approximately 2000 mixed-age ewes were obtained from a Merino nucleus flock. Genome-wide association studies were performed utilizing single-step Bayesian analysis. For wool traits, a total of 35 genomic windows surpassed the significance threshold (PVE > 0.25%). The proportion of the total additive genetic variance explained by those windows was 4.85 and 9.06% for FD and CFW, respectively. There were 42 windows significantly associated with LWM, which collectively explained 43.2% of the additive genetic variance. For BCS, 22 relevant windows accounted for more than 40% of the additive genetic variance, whereas for the reproduction traits, 53 genomic windows (24 and 29 for PR and LP, respectively) reached the suggestive threshold of 0.25% of the PVE. Within the top 10 windows for each trait, we identified several genes showing potential associations with the wool (e.g., IGF-1, TGFB2R, PRKCA), live weight (e.g., CAST, LAP3, MED28, HERC6), body condition score (e.g., CDH10, TMC2, SIRPA, CPXM1) or reproduction traits (e.g., ADCY1, LEPR, GHR, LPAR2) of the mixed-age ewes. Copyright: © 2023 by the authors 653 $aBody condition score 653 $aFiber diameter 653 $aGene 653 $aGWAS 653 $aReproduction 653 $aSheep 700 1 $aGARRICK , D. J. 700 1 $aBLAIR, H. T. 700 1 $aVERA, B. 700 1 $aCIAPPESONI, G. 700 1 $aKENYON, P. R. 773 $tGenes, 2023, volume 14, issue 1, article 167. OPEN ACCESS. doi: https://doi.org/10.3390/genes14010167
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